RESPIRATION-(higher level)

 

Glycolysis Glycolysis Animations

 

The Krebs Cycle Electron Transport Aerobic Respiration Animations

 

Using ATP Anaerobic Respiration Lactic Acid

 

Alcohol Fermentation Anaerobic Respiration Summary

 

Biotechnology Immobilised Cells

 

 

Please read through the Respiration-ordinary level web page to get an introduction to this topic.

 

AEROBIC RESPIRATION

 

STAGE 1-GLYCOLYSIS

 

Glycolysis takes place in the cytosol. The cytosol is the clear portion of the cytoplasm. Here is where the enzymes needed for stage 1 are located. This process does not need oxygen.

The process of glycolysis functions to split a glucose molecule into two 3-carbon molecules called pyruvic acid (C3H4O3). Pyruvic acid is a neutral particle. It may also be found as a negatively charged ion called pyruvate. The energy released is used to form 2 ATP molecules + water + 2 NADH molecules.

As stated previously in the ordinary level web page, if there is no oxygen available then anaerobic respiration takes place.

IF OXYGEN IS PRESENT:

If oxygen is present the pyruvic acid enters the mitochondria. (Recall that glycolysis occurs in the cytosol of the cell.) Here it loses a carbon dioxide molecule and forms a 2-carbon molecule called acetyl coenzyme A (CoA). While forming acetyl CoA it loses two high energy electrons and a proton. These are used to form NADH. The NADH then goes to the electron transport system. (will be discussed later) If oxygen is not present anaerobic respiration takes place.

 

Click here to view an interactive animation of glycolysis

Click here to view an animation of glycolysis

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STAGE 2-The KREBS CYCLE:

 

The acetyl CoA now undergoes changes called the Krebs Cycle. The acetyl CoA is broken down to carbon

dioxide and protons (H+). The energy contained in the acetyl CoA is released in the form of high energy electrons.

The electrons and protons are picked up by NAD+ to form NADH. The NADH goes into the electron transport system. (will be discussed later) Also phosphorylation occurs where a phosphate group is added to ADP to form ATP.

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STAGE 2- THE ELECTRON TRANSPORT SYSTEM

 

The electron transport system happens in the inner membrane of the mitochondria. Remember that Stage 1 (glycolysis) occurs in the cytosol of the cell.

The electron transport system is composed of a series of proteins that transfer NADH from protein to protein.

With each transfer to a new protein some of the energy in the NADH is released to form ATP. This is done by oxidative phosphorylation. Refer to the Energy for Life web page to review oxidative phosphorylation.

At the end of the electron transport system electrons are used to combine hydrogen with oxygen to form water. If there is no oxygen present the electron transport system cannot operate and therefore the organism will die as a result of the lack of energy (ATP) production.

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3 Animations of electron transport in Mitochondria:

 

Animation 1:

1. Protons are moved across the membrane, from the cytosol to the intermembrane space.

2. Electrons are transported along the membrane, through a series of protein carriers depicted as Q and cyt C in the diagram.

3. Oxygen is the final electron acceptor, combining with electrons and H+ ions to produce water.

 

 

 

Animation 2:

Recall that a molecule is reduced when it gains and electron and it is oxydized when it loses an electron.

 

Animation 3:

NADH and carries protons (H+) and electrons (e-) to the electron transport chain located in the membrane. At the end of the electron transport system, two protons, two electrons, and half of an oxygen molecule combine to form water.

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Animation of ATP formation

 

ADP combines with a phosphate group to form ATP. The ATP Synthase is the enzyme used in this process.

 

Click here to view an animation of electron transfer and ATP formation

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USING ATP IN THE CELL

 

To obtain energy to do cellular work, the cell enzymatically removes the third phosphate from ATP thus releasing the stored energy and forming ADP and phosphate once again.

 

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Aerobic Respiration Links

 

Aerobic Respiration Animation

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ANAEROBIC RESPIRATION (ordinary level)

In anaerobic respiration oxygen is not needed although it can take place in the presence of oxygen. In anaerobic respiration glycolysis takes place. The 3-carbon molecules (pyruvic acid) are then converted into other products. Anaerobic respiration is also known as fermentation.

Two products of anaerobic respiration are lactic acid and alcohol.

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Lactic Acid

Lactic acid is produced in some bacteria, fungi, and muscle tissue.

Pyruvic Acid is changed into lactic acid + small amount of energy

Pyruvic Acid is changed to Lactic Acid + energy (ATP)

Pyruvic AcidLactic Acid

Examples of Lactic Acid:

Bacteria causing milk to sour

Bacteria change cabbage to sauerkraut

Bacteria changing dairy products to cheese and yoghurt

Build up of lactic acid in muscles (because of lack of oxygen) causing cramps and stiffness

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ALCOHOL FERMENTATION

Alcohol fermentation occurs in some bacteria, yeasts, and in plants deprived of oxygen. Again the pyruvic acid formed by glycolysis is changed. Here it is changed into ethanol ethyl alcohol) and carbondioxide.

Pyruvic Acid is changed into ethanol + small amount of energy

Pyruvic Acid is changed to Alcohol + Carbon Dioxide + energy (ATP)

Pyruvic Acid EthanolCarbon Dioxide

 

 

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Uses of Alcohol Fermentation

Baking: the carbon dioxide causes the dough to rise

Beer and Wine production

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Biotechnology

(See batch processing and continuous flow processing by clicking on the Bacteria Webpage on the Homepage)

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Immobilised Cells

Just as enzymes are immobilised for commercial uses bacteria, fungi, and yeasts are also immobilised for production of products. See the enzyme webpage to review immobilisation techniques.

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